[
  {"id": 1, "parent_plasticdb_id": "00003", "parent_protein_id": 3, "variant_name": "FAST-PETase", "variant_type": "engineered", "description": "Functional, active, stable and tolerant PETase: five mutations vs WT PETase (S121E/D186H/R224Q/N233K/R280A). Highest overall PET-hydrolytic activity at 30-50 C of all enzymes tested; degrades untreated postconsumer PET completely within 1 week at 50 C.", "mutations": "S121E; D186H; R224Q; N233K; R280A", "engineering_method": "machine learning (MutCompute) guided design", "performance": "At 40 and 50 C, 2.4- and 38-fold higher activity than ThermoPETase; releases 33.8 mM PET monomers from amorphous gf-PET in 96 h at 50 C (highest of all mutants/temperatures tested).", "kinetics": null, "properties": null, "accession": "PBVPYT", "seq_id": "PBVPYT", "sequence": "MNFPRASRLMQAAVLGGLMAVSAAATAQTNPYARGPNPTAASLEASAGPFTVRSFTVSRPSGYGAGTVYYPTNAGGTVGAIAIVPGYTARQSSIKWWGPRLASHGFVVITIDTNSTLDQPESRSSQQMAALRQVASLNGTSSSPIYGKVDTARMGVMGWSMGGGGSLISAANNPSLKAAAPQAPWHSSTNFSSVTVPTLIFACENDSIAPVNSSALPIYDSMSQNAKQFLEIKGGSHSCANSGNSNQALIGKKGVAWMKRFMDNDTRYSTFACENPNSTAVSDFRTANCS"},
  {"id": 2, "parent_plasticdb_id": "00003", "parent_protein_id": 3, "variant_name": "ThermoPETase", "variant_type": "engineered", "description": "Thermostabilized PETase scaffold (S121E/D186H/R280A) used as the background for FAST-PETase.", "mutations": "S121E; D186H; R280A", "engineering_method": "rational protein engineering (Son et al. 2019)", "performance": "Higher thermostability than WT PETase; FAST-PETase (built on this scaffold) shows 2.4- and 38-fold higher activity at 40 and 50 C.", "kinetics": null, "properties": null, "accession": "PB6CC8", "seq_id": "PB6CC8", "sequence": "MNFPRASRLMQAAVLGGLMAVSAAATAQTNPYARGPNPTAASLEASAGPFTVRSFTVSRPSGYGAGTVYYPTNAGGTVGAIAIVPGYTARQSSIKWWGPRLASHGFVVITIDTNSTLDQPESRSSQQMAALRQVASLNGTSSSPIYGKVDTARMGVMGWSMGGGGSLISAANNPSLKAAAPQAPWHSSTNFSSVTVPTLIFACENDSIAPVNSSALPIYDSMSRNAKQFLEINGGSHSCANSGNSNQALIGKKGVAWMKRFMDNDTRYSTFACENPNSTAVSDFRTANCS"},
  {"id": 3, "parent_plasticdb_id": "00003", "parent_protein_id": 3, "variant_name": "DuraPETase", "variant_type": "engineered", "description": "Computationally redesigned durable PETase scaffold (10 mutations).", "mutations": "L117F; Q119Y; T140D; W159H; G165A; I168R; A180I; S188Q; S214H; R280A", "engineering_method": "computational redesign (GRAPE strategy, Cui et al. 2021)", "performance": "High thermostability but relatively low activity at mild temperatures (30-50 C).", "kinetics": null, "properties": null, "accession": "PBN74C", "seq_id": "PBN74C", "sequence": "MNFPRASRLMQAAVLGGLMAVSAAATAQTNPYARGPNPTAASLEASAGPFTVRSFTVSRPSGYGAGTVYYPTNAGGTVGAIAIVPGYTARQSSIKWWGPRLASHGFVVITIDTNSTFDYPSSRSSQQMAALRQVASLNGDSSSPIYGKVDTARMGVMGHSMGGGASLRSAANNPSLKAAIPQAPWDSQTNFSSVTVPTLIFACENDSIAPVNSHALPIYDSMSRNAKQFLEINGGSHSCANSGNSNQALIGKKGVAWMKRFMDNDTRYSTFACENPNSTAVSDFRTANCS"},
  {"id": 4, "parent_plasticdb_id": "00004", "parent_protein_id": 4, "variant_name": "ICCM", "variant_type": "engineered", "description": "Engineered LCC (F243I/D238C/S283C/N246M) - the most active PET-hydrolysing LCC variant reported by Tournier et al.; a disulfide-bridge stabilized, benchmark thermophilic PET depolymerase.", "mutations": "D238C; F243I; N246M; S283C", "engineering_method": "rational engineering (Tournier et al. 2020)", "performance": "Optimum near 72 C; at its optimum still 1.5-fold lower than FAST-PETase at 50 C on pc-PET.", "kinetics": null, "properties": null, "accession": "PBR42U", "seq_id": "PBR42U", "sequence": "MDGVLWRVRTAALMAALLALAAWALVWASPSVEAQSNPYQRGPNPTRSALTADGPFSVATYTVSRLSVSGFGGGVIYYPTGTSLTFGGIAMSPGYTADASSLAWLGRRLASHGFVVLVINTNSRFDYPDSRASQLSAALNYLRTSSPSAVRARLDANRLAVAGHSMGGGGTLRIAEQNPSLKAAVPLTPWHTDKTFNTSVPVLIVGAEADTVAPVSQHAIPFYQNLPSTTPKVYVELCNASHIAPMSNNAAISVYTISWMKLWVDNDTRYRQFLCNVNDPALCDFRTNNRHCQ"},
  {"id": 5, "parent_plasticdb_id": "00004", "parent_protein_id": 4, "variant_name": "LCC D238K", "variant_type": "engineered", "description": "Lysine-mutation (LM) variant of LCC introducing the N233K-equivalent lysine mutation.", "mutations": "D238K", "engineering_method": "site-directed mutagenesis (lysine mutation, portability test)", "performance": "Tm increased ~7 C over WT LCC; higher gf-PET activity than WT LCC.", "kinetics": null, "properties": "[{\"optimal_temperature\": null, \"optimal_ph\": null, \"tm\": 96.277, \"t50\": null, \"half_life\": null, \"half_life_temperature\": null, \"assay_notes\": \"Tm ~96.3 C by DSC, +7 C vs WT LCC (Fig. 2a)\"}]", "accession": "PB6TK6", "seq_id": "PB6TK6", "sequence": "MDGVLWRVRTAALMAALLALAAWALVWASPSVEAQSNPYQRGPNPTRSALTADGPFSVATYTVSRLSVSGFGGGVIYYPTGTSLTFGGIAMSPGYTADASSLAWLGRRLASHGFVVLVINTNSRFDYPDSRASQLSAALNYLRTSSPSAVRARLDANRLAVAGHSMGGGGTLRIAEQNPSLKAAVPLTPWHTDKTFNTSVPVLIVGAEADTVAPVSQHAIPFYQNLPSTTPKVYVELKNASHFAPNSNNAAISVYTISWMKLWVDNDTRYRQFLCNVNDPALSDFRTNNRHCQ"},
  {"id": 6, "parent_plasticdb_id": "00005", "parent_protein_id": 5, "variant_name": "Cut190 D250K", "variant_type": "engineered", "description": "Lysine-mutation (LM) variant of Cut190.", "mutations": "D250K", "engineering_method": "site-directed mutagenesis (lysine mutation, portability test)", "performance": "Tm increased ~7 C over WT Cut190; higher gf-PET activity than WT Cut190.", "kinetics": null, "properties": "[{\"optimal_temperature\": null, \"optimal_ph\": null, \"tm\": 62.527, \"t50\": null, \"half_life\": null, \"half_life_temperature\": null, \"assay_notes\": \"Tm ~62.5 C by DSC, +7 C vs WT Cut190 (Fig. 2a)\"}]", "accession": "PBBJHM", "seq_id": "PBBJHM", "sequence": "MRIRRQAGTGARASMARAIGVMTTALAVLVGAVGGVAGAEVSTAQDNPYERGPDPTEDSIEAIRGPFSVATERVSSFASGFGGGTIYYPRETDEGTFGAVAVAPGFTASQGSMSWYGERVASQGFIVFTIDTNTRLDQPGQRGRQLLAALDYLVERSDRKVRERLDPNRLAVMGHSMGGGGSLEATVMRPSLKASIPLTPWNLDKTWGQVQVPTFIIGAELDTIASVRTHAKPFYESLPSSLPKAYMELKGATHFAPNIPNTTIAKYVISWLKRFVDEDTRYSQFLCPNPTDRAIEEYRSTCPY"},
  {"id": 7, "parent_plasticdb_id": "00004", "parent_protein_id": 4, "variant_name": "ICCM C238K/C283S", "variant_type": "engineered", "description": "Lysine-mutation variant built on ICCM: C238K reverts the ICCM D238C disulfide cysteine to lysine and C283S reverts S283C to serine (net vs WT LCC: D238K/F243I/N246M).", "mutations": "D238K; F243I; N246M", "engineering_method": "site-directed mutagenesis (lysine mutation, portability test)", "performance": "Tm 96.3 C (-3.7 C vs ICCM) but higher gf-PET activity than ICCM.", "kinetics": null, "properties": "[{\"optimal_temperature\": null, \"optimal_ph\": null, \"tm\": 96.337, \"t50\": null, \"half_life\": null, \"half_life_temperature\": null, \"assay_notes\": \"Tm 96.3 C by DSC, -3.7 C vs ICCM (Fig. 2a)\"}]", "accession": "PBZQ49", "seq_id": "PBZQ49", "sequence": "MDGVLWRVRTAALMAALLALAAWALVWASPSVEAQSNPYQRGPNPTRSALTADGPFSVATYTVSRLSVSGFGGGVIYYPTGTSLTFGGIAMSPGYTADASSLAWLGRRLASHGFVVLVINTNSRFDYPDSRASQLSAALNYLRTSSPSAVRARLDANRLAVAGHSMGGGGTLRIAEQNPSLKAAVPLTPWHTDKTFNTSVPVLIVGAEADTVAPVSQHAIPFYQNLPSTTPKVYVELKNASHIAPMSNNAAISVYTISWMKLWVDNDTRYRQFLCNVNDPALSDFRTNNRHCQ"}
]